PCR反應(yīng)體系包括10×PCR buffer(含Mg2+) 5μl、Taq酶(2U/μl)1μl、dNTPs(10mmol/L)1μl、上下游引物(10mmol/L)各1μl、DNA模板(50ng/μl)1μl,加ddH2O至50μl。PCR反應(yīng)在Gene AmpPCR System 9 600 (Perkin Elmer,USA)上進(jìn)行。PCR反應(yīng)程序?yàn)?94℃ 5min; 94℃ 30s,48~56℃ 30s,72℃ (共 414 字) [閱讀本文] >>